Persistent infection of Friend erythroleukemia cells with vaccinia virus.
نویسندگان
چکیده
منابع مشابه
Persistent infection of Friend erythroleukemia cells with vaccinia virus.
Cultures of murine Friend erythroleukemia (FL) cells, which are chronically infected with leukemia virus, were inoculated with vaccinia virus. The yield of vaccinia virus was determined by assaying plaque-forming units in mouse L2 cells, and the yield of leukemia virus was determined by measuring reverse transcriptase (RNA-dependent DNA nucleotidyltransferase) activity released into the culture...
متن کاملSynthesis of murine leukemia virus proteins differentiating Friend erythroleukemia cells.
In agreement with previous observations, the Friend 745 line of virus-induced erythroleukemia cells can be induced by dimethyl sulfoxide to synthesize hemoglobin and to increase simultaneously their content of morpho logically recognizable virus particles, especially in cytoplasmic vesicles. In apparent contrast to the electron microscopic results, the cellular content of the major murine leuke...
متن کاملInactivation of Friend erythroleukemia virus and Friend virus-transformed cells by merocyanine 540-mediated photosensitization.
The Friend virus complex was used as a model to study the effects of merocyanine 540 (MC 540)-mediated photosensitization on enveloped viruses. Simultaneous exposure to the lipophilic dye MC 540 and white light inactivated cell-free virus, cell-associated virus, and virus-transformed cells. When used under experimental conditions that are known to preserve most mature blood cells, at least some...
متن کاملInduction of the early stages of Friend erythroleukemia with helper-free Friend spleen focus-forming virus.
The polycythemia-inducing strain of Friend virus (FV-P) causes a multistage erythroleukemia in susceptible mice. FV-P is a complex of two viruses, a replication-competent virus [Friend murine leukemia virus (F-MuLV)] and a replication-defective spleen focus-forming virus (SFFVp). We have addressed directly the role of SFFVp in the induction of the early stages of Friend disease by constructing ...
متن کاملCharacterization of leukemogenic virus produced by a new line of Friend erythroleukemia virus-transformed cells.
A new cell line designated SQ-A was established from the spleen of a leukemic DBA/2J mouse inoculated with the anemic strain of Friend erythroleukemia virus (FLV-A). The cells are similar in morphology, growth pattern, and tumorigenicity to our prototype erythroleukemia line 5-86 but are more sensitive to the cytotoxic effects of inducers of differentiation. The virus produced by SQ-A cells ind...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1982
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.79.15.4805